This course in microbiology consists of three one-hour lectures and one three-hour laboratory session each week. The lectures describe the basic biology of microbial life, with emphasis on bacteria (Domain Bacteria and Domain Archaea) and information on eukaryotic microbes (protists, fungi, and unicellular algae) of Domain Eukarya.
Brock Biology of Microorganisms, M.T. Madigan, J.W. Martinko, P.V. Dunlap, and D.P. Clark, 13th edition, Pearson/Benjamin Cummings, 2009.
Laboratory manual (required):
Benson's Microbiological Applications: Laboratory Manual in General Microbiology, short version, A.E. Brown, 12th edition, McGraw-Hill, 2009.
Grades for the course are based on four exams from lecture material together with assessments of practical skills and laboratory reports from the laboratory sessions.
The course is required for the Microbiology concentration, and it is appropriate for the Biology and the Cell and Molecular Biology concentrations.
Lecture material in the course is divided into four approximately equal sections.
- The first section examines the historical origins of microbiology as a science; bacterial and eukaryal cell structure and function, and bacterial nutrition, cultivation, and carbon and energy metabolsim.
- The second section addresses molecular biology of bacteria, including DNA replication, transcription and translation, regulation of gene expression, genomics, and bacterial viruses.
- The third section of the course covers microbial evolution, metabolic and ecological diversity, nutrient cycles, and symbiotic interactions.
- The final section of the course describes medically related topics, including host-pathogen interactions, epidemiology, immunology, and bacterial, viral, protozoan, and fungal pathogenesis.
The laboratory sessions are designed to guide the development of skill in fundamental aspects of microbiological work, including microscopy, aseptic and pure-culture techniques, experimental manipulation of bacteria, microbial enrichment, and isolation and characterization of bacteria.