The course introduces selected experimental methods used to solve problems in modern biochemistry. The focus is on quantitative methods, i.e. methods providing "numbers" rather than "yes/no" answers. For each method, the course first lays out the necessary theoretical background and presents typical instrumentations and procedures. We then discuss applications, i.e., how the method is used to address a specific biochemical question, as well as its limitations. The course also provides examples from the scientific literature of the successful (and sometimes less successful) use of these methods.
Two written exams; participation in class discussions; written homework.
Biology, CMB, and Microbiology undergraduates; graduate students in molecular biology, cell biology, and biochemistry; and others interested in pursuing a research career in the biosciences.
3 hours lecture weekly