This laboratory course encompasses a broad spectrum of common methods and theory, in both prokaryotic and eukaryotic cells, that are essential to the Molecular, Cellular & Developmental Biology major.
Techniques include microscopy, bacterial culture, transformation, plasmid construction, gel electrophoresis, polymerase chain reaction, DNA sequencing, tissue culture, transfection, cellular organelle isolation, column chromatography, Western blotting, immunohistochemistry, and CRISPR-Cas-9 genetic engineering.
This course can be used to satisfy requirements for the Molecular, Cellular & Developmental Biology major and the Biology major.
Course Requirements:
Grades are based on two exams, laboratory quizzes, and a term-long grant proposal project that may be completed individually or in a self-organized group, several small projects that are extensions of the lab material, and oral presentations as a group in lab.
Class Format:
The 50-minute lecture each week is used to introduce the methodology and techniques that will be encountered in the lab. Two weekly lab sessions are arranged in a project-based format.