Agilent Q-TOF  HPLC-MS

The Agilent Q-TOF has an ultra-high pressure HPLC system with a binary pump, an autosampler with thermostatic temperature control, two column modules capable of heating and cooling, and a diode array detector.  The mass spectrometer is capable of being operated in either MS mode, or MS-MS mode.  Compounds can be run by Electrospray or APCI, in both positive and negative ion mode.
Small molecules can be mass measured to an accuracy of within 2 ppm in MS mode.  In MS-MS mode, fragments can be mass measured to an accuracy of within 5 ppm. In addition to being able to do exact mass measurements on small molecules, this system also excels at running peptides, and also intact proteins.
Q-TOF Booklet (pdf)

tof Students who have a demonstrated need for the unique capabilities of this instrument can be trained to run their own samples.  The  usual training session taking 4 hours.  Up to four students can attend the same training session to divide the cost.  Please see billing rates.

Please see billing rates for non-routine samples submitted to us to be run on the Q-TOF and student use of the instrument.

Please note that the Jacob lab, the Bardwell lab, and the Walter lab have priority in scheduling time on Thursday through Sunday of each week.

Reserve a time on this machine using the CTools calendar.
If you are outside the University of Michigan, contact Jim Windak to arrange time.

 

Bruker AutoFlex Speed MALDI-TOF

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The Bruker AutoFlex Speed is a MALDI-TOF instrument capable of doing both Linear and Reflectron modes, with either positive or negative ion detection.  It is suitable for analysis of peptides, intact proteins, and polymers.
The instrument accepts a wide variety of Bruker sample plates, from the smaller 96 well plate to the larger 384 well plate.  The software includes the Bruker poly tools package for polymer data analysis

Reserve a time on this machine using the CTools calendar.
If you are outside UMich, contact Jim Windak to arrange time.

Shimadzu QP-2010 GCMS

traceMS

The Shimadzu QP-2010 GC/MS is available for hands-on use by graduate students who need to run their own samples. It has a quadrupole mass spectrometer capable of unit mass resolution. The gas chromatograph is equipped with an autosampler and an auto-injector.  The GC contains a 30 meter long DB-5 column with a 0.25 mm I.D.  The injector can be used in either split or splitless modes.

 Students who have a need to use this instrument should e-mail Jim Windak to schedule a 30-minute training session. You will also need to provide an account number to use for billing your access time. Please see billing rates.

Shimadzu GCMS user’s booklet.

Reserve a time on this machine using the CTools calendar.
If you are outside UMich, contact Jim Windak to arrange time.

 

Micromass TofSpec-2E Matrix-Assisted, Laser-Desorption Time-of-Flight Mass Spectrometer

tof

The Micromass TofSpec-2E is a MALDI-TOF mass spectrometer that is capable of operating in either Linear or Reflectron modes. It can be used in reflectron mode with unit mass resolution up to around 5000 daltons, to analyze peptides or small polymers. The instrument can also be operated in low resolution linear mode to analyze proteins or large polymers. It also has a high mass, post acceleration dynode detector, which can be used to enhance weak mass signals from very large molecules over 100,000 daltons. The instrument uses a 96 spot plate, and can be set up to do automatic data acquisition on each spot within the plate.
This instrument is available for student hands-on use. If you need to use this instrument, please e-mail Jim Windak to schedule a training session.  Prior to the training session, you should print out and read the TofSpec User's Booklet. You will also need to purchase a sample plate from Micromass for your own use.  Please see billing rates.

Reserve a time on this machine using the CTools calendar.
If you are outside University of Michigan, contact Jim Windak to arrange time.

Micromass LCT Time-of-Flight mass spectrometer with Electrospray and APCI

lct

The Micromass LCT is a benchtop time-of-flight mass spectrometer with Electrospray and APCI ionization modes. It has an HPLC pump with with a Rheodyne loop injector, and also a syringe pump for sample introduction. It is capable of operating in either positive ion or negative ion mode. It is generally used for organic molecules that have a mass range of 100-1500 daltons. However, it can also be used to analyze larger molecules such as peptides or proteins. It has transform software for deconvoluting multiply charged ion peaks of large molecules, such as proteins. It can also do exact mass measurements on small molecules, generally to within 5 ppm for masses in the range of 400-1000 daltons.
The instrument is available for student hands-on use. To use the instrument, please e-mail Jim Windak to schedule a training session. You will need a six-digit short code account number to use for billing your access time.  Prior to the training session, you should print out and read the LCT User's Booklet.  Please see billing rates.

 

Micromass AutoSpec Ultima Magnetic sector mass spectrometer

auto

The Micromass Ultima is a high resolution magnetic sector mass spectrometer that is capable of analyzing small organic molecules using EI, CI or Electrospray. It is capable of doing both low resolution, full mass range scans, as well as high resolution, exact mass measurements. The exact mass measurements are generally accurate to within a few PPM on positive ions for masses up to 1500 daltons.
Samples to be analyzed on this instrument are submitted along with a Sample Submission Form. Turn-around times are generally within 2-3 working days.  Please see billing rates.


VG (Micromass) 70-250-S Magnetic sector mass spectrometer

The VG 70-250-S mass spectrometer is a high resolution, magnetic sector instrument that is capable of analyzing small organic molecules by EI, CI, or FAB. It is capable of doing both low resolution, full mass range scans, as well as high resolution, exact mass measurements. The exact mass measurements are generally accurate to within 5 PPM on positive ions for masses under 1000 Da.
Samples to be analyzed on this instrument are submitted along with a Sample Submission Form. Turn-around times are generally within 2-3 working days. Please see billing rates.