GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking

Author(s): Yi Xiang, Yanzhuang Wang


Description:

In vitro studies have suggested that Golgi stack formation involves two homologous peripheral Golgi proteins, GRASP65 and GRASP55, which localize to the cis and medial-trans cisternae, respectively. However, no mechanism has been provided on how these two GRASP proteins work together to stack Golgi cisternae. Here, we show that depletion of either GRASP55 or GRASP65 by siRNA reduces the number of cisternae per Golgi stack, whereas simultaneous knockdown of both GRASP proteins leads to disassembly of the entire stack




Publication Information:

Name of Periodical: J. Cell Biol.

Volume Number: 188

Issue Number: 2

Year of Publication: 2010