The Rab GTPase RabA4d Regulates Pollen Tube Tip Growth in Arabidopsis thaliana
Author(s): Amy Szumlanski, Erik Nielsen
During reproduction in flowering plants, pollen grains form a tube that grows in a polarized fashion through the female tissues to eventually fertilize the egg cell. These highly polarized pollen tubes have a rapid rate of growth that is supported by a tip-focused delivery of membrane and cell wall components. To gain a better understanding of how this growth is regulated, we investigated the function RABA4D, a member of the Arabidopsis thaliana RabA4 subfamily of Rab GTPase proteins. Here, we show that RABA4D was expressed in a pollen-specific manner and that enhanced yellow fluorescent protein (EYFP)-RabA4d-labeled membrane compartments localized to the tips of growing pollen tubes.
Name of Periodical: Plant Cell
Volume Number: 21
Issue Number: 2
Year of Publication: 2009